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2a3 cell line (ATCC)

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ATCC 2a3 cell line

(A-D) Venn diagrams of differentially expressed genes in FaDu (A-B) and <t>2A3</t> (C-D) cell lines. Venn diagrams illustrate the number of significantly regulated genes under three conditions: hypoxia (green), irradiation (yellow), and the combination of hypoxia and irradiation (blue). Numbers in the overlapping areas indicate genes commonly regulated under the corresponding conditions. Genes were considered differentially expressed if they had an adjusted p-value (padj) <0.1. (E) Biological processes differentially regulated in FaDu and 2A3 HNSCC cell lines under hypoxia and combined hypoxia with gamma irradiation. The figure illustrates biological processes significantly upregulated (red) or downregulated (green) in FaDu and 2A3 cells in response to hypoxia alone or in combination with gamma radiation. The analysis was performed using the WebGestalt 2024 platform and included only genes that were consistently detected across all three independent biological replicates and met the inclusion criteria of adjusted p < 0.1 and |log 2 FC| ≥ 2.

2a3 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2a3 cell line/product/ATCC
Average 94 stars, based on 39 article reviews

2a3 cell line - by Bioz Stars, 2026-03

94/100 stars

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1) Product Images from "HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation"

Article Title: HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation

Journal: bioRxiv

doi: 10.1101/2025.10.26.684626

(A-D) Venn diagrams of differentially expressed genes in FaDu (A-B) and 2A3 (C-D) cell lines. Venn diagrams illustrate the number of significantly regulated genes under three conditions: hypoxia (green), irradiation (yellow), and the combination of hypoxia and irradiation (blue). Numbers in the overlapping areas indicate genes commonly regulated under the corresponding conditions. Genes were considered differentially expressed if they had an adjusted p-value (padj) <0.1. (E) Biological processes differentially regulated in FaDu and 2A3 HNSCC cell lines under hypoxia and combined hypoxia with gamma irradiation. The figure illustrates biological processes significantly upregulated (red) or downregulated (green) in FaDu and 2A3 cells in response to hypoxia alone or in combination with gamma radiation. The analysis was performed using the WebGestalt 2024 platform and included only genes that were consistently detected across all three independent biological replicates and met the inclusion criteria of adjusted p < 0.1 and |log 2 FC| ≥ 2.

Figure Legend Snippet: (A-D) Venn diagrams of differentially expressed genes in FaDu (A-B) and 2A3 (C-D) cell lines. Venn diagrams illustrate the number of significantly regulated genes under three conditions: hypoxia (green), irradiation (yellow), and the combination of hypoxia and irradiation (blue). Numbers in the overlapping areas indicate genes commonly regulated under the corresponding conditions. Genes were considered differentially expressed if they had an adjusted p-value (padj) <0.1. (E) Biological processes differentially regulated in FaDu and 2A3 HNSCC cell lines under hypoxia and combined hypoxia with gamma irradiation. The figure illustrates biological processes significantly upregulated (red) or downregulated (green) in FaDu and 2A3 cells in response to hypoxia alone or in combination with gamma radiation. The analysis was performed using the WebGestalt 2024 platform and included only genes that were consistently detected across all three independent biological replicates and met the inclusion criteria of adjusted p < 0.1 and |log 2 FC| ≥ 2.

Techniques Used: Irradiation

(A) FaDu, 2A3, and Detroit-562 cell lines were cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions. Cell numbers were determined 48 and 72 hours after plating, and doubling times were calculated as described in Methods. Data represent the mean ± SD from four independent experiments (n = 4). Statistical analysis was performed using an unpaired t-test (**p < 0.01). (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. Cell cycle distribution was analyzed 24 hours after 6 Gy gamma-irradiation in (C) FaDu, (D) 2A3, and (E) Detroit-562 cells cultured under normoxic or hypoxic (1% O 2 ) conditions. Results are presented as the percentage of cells in G0/1, S, or G2/M phases. Data represent the mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Figure Legend Snippet: (A) FaDu, 2A3, and Detroit-562 cell lines were cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions. Cell numbers were determined 48 and 72 hours after plating, and doubling times were calculated as described in Methods. Data represent the mean ± SD from four independent experiments (n = 4). Statistical analysis was performed using an unpaired t-test (**p < 0.01). (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. Cell cycle distribution was analyzed 24 hours after 6 Gy gamma-irradiation in (C) FaDu, (D) 2A3, and (E) Detroit-562 cells cultured under normoxic or hypoxic (1% O 2 ) conditions. Results are presented as the percentage of cells in G0/1, S, or G2/M phases. Data represent the mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Techniques Used: Cell Culture, Software, Irradiation

(A) Cleaved caspase-3 levels in FaDu, 2A3, and Detroit-562 cells cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Results are shown as fold change relative to non-irradiated normoxic controls. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (C) Representative western blots of N-cadherin, E-cadherin, and vimentin in FaDu and 2A3 cells. (D) Quantification of protein levels expressed as mean optical density (O.D.) ± SD, normalized to β-actin, in FaDu and 2A3 cells under normoxic or hypoxic conditions. (E–G) Effects of hypoxia and irradiation on N-cadherin, E-cadherin, and vimentin levels in FaDu and 2A3 cells under normoxic and hypoxic conditions. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: unpaired t-test (*p < 0.05).

Figure Legend Snippet: (A) Cleaved caspase-3 levels in FaDu, 2A3, and Detroit-562 cells cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Results are shown as fold change relative to non-irradiated normoxic controls. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (C) Representative western blots of N-cadherin, E-cadherin, and vimentin in FaDu and 2A3 cells. (D) Quantification of protein levels expressed as mean optical density (O.D.) ± SD, normalized to β-actin, in FaDu and 2A3 cells under normoxic or hypoxic conditions. (E–G) Effects of hypoxia and irradiation on N-cadherin, E-cadherin, and vimentin levels in FaDu and 2A3 cells under normoxic and hypoxic conditions. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: unpaired t-test (*p < 0.05).

Techniques Used: Cell Culture, Irradiation, Software, Western Blot

(A) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (B) Cytokine/chemokine production in response to hypoxia, gamma-irradiation, and their combination. Conditioned media from FaDu, 2A3, and Detroit-562 cells were collected 48 hours after gamma-irradiation under normoxic or hypoxic conditions. Media from three independent experiments per cell line were pooled for analysis. Results are presented as z-scores of log 10 -transformed normalized data. n.d. = not detected. (C) Cell numbers of FaDu, 2A3, and Detroit-562 cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Data represent mean ± SD from four independent experiments (n = 4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. Levels of IL-8 (D), MIF (E), and Serpin E1 (F) in supernatants from FaDu, 2A3, and Detroit-562 cells cultured under normoxic or hypoxic conditions, assessed 48 hours after gamma-irradiation. Concentrations were normalized to cell numbers per condition. Data represent mean ± SD from three to four independent experiments (n = 3–4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Figure Legend Snippet: (A) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (B) Cytokine/chemokine production in response to hypoxia, gamma-irradiation, and their combination. Conditioned media from FaDu, 2A3, and Detroit-562 cells were collected 48 hours after gamma-irradiation under normoxic or hypoxic conditions. Media from three independent experiments per cell line were pooled for analysis. Results are presented as z-scores of log 10 -transformed normalized data. n.d. = not detected. (C) Cell numbers of FaDu, 2A3, and Detroit-562 cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Data represent mean ± SD from four independent experiments (n = 4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. Levels of IL-8 (D), MIF (E), and Serpin E1 (F) in supernatants from FaDu, 2A3, and Detroit-562 cells cultured under normoxic or hypoxic conditions, assessed 48 hours after gamma-irradiation. Concentrations were normalized to cell numbers per condition. Data represent mean ± SD from three to four independent experiments (n = 3–4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Techniques Used: Software, Irradiation, Transformation Assay, Cell Culture

Image Search Results

Journal: bioRxiv

Article Title: HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation

doi: 10.1101/2025.10.26.684626

Figure Lengend Snippet: (A-D) Venn diagrams of differentially expressed genes in FaDu (A-B) and 2A3 (C-D) cell lines. Venn diagrams illustrate the number of significantly regulated genes under three conditions: hypoxia (green), irradiation (yellow), and the combination of hypoxia and irradiation (blue). Numbers in the overlapping areas indicate genes commonly regulated under the corresponding conditions. Genes were considered differentially expressed if they had an adjusted p-value (padj) <0.1. (E) Biological processes differentially regulated in FaDu and 2A3 HNSCC cell lines under hypoxia and combined hypoxia with gamma irradiation. The figure illustrates biological processes significantly upregulated (red) or downregulated (green) in FaDu and 2A3 cells in response to hypoxia alone or in combination with gamma radiation. The analysis was performed using the WebGestalt 2024 platform and included only genes that were consistently detected across all three independent biological replicates and met the inclusion criteria of adjusted p < 0.1 and |log 2 FC| ≥ 2.

Article Snippet: The 2A3 cell line (CRL-3212, ATCC, Manassas, VA, USA) was generated by transfecting FaDu cells with the E6 and E7 genes of HPV-16.

Techniques: Irradiation

Journal: bioRxiv

Article Title: HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation

doi: 10.1101/2025.10.26.684626

Figure Lengend Snippet: (A) FaDu, 2A3, and Detroit-562 cell lines were cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions. Cell numbers were determined 48 and 72 hours after plating, and doubling times were calculated as described in Methods. Data represent the mean ± SD from four independent experiments (n = 4). Statistical analysis was performed using an unpaired t-test (**p < 0.01). (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. Cell cycle distribution was analyzed 24 hours after 6 Gy gamma-irradiation in (C) FaDu, (D) 2A3, and (E) Detroit-562 cells cultured under normoxic or hypoxic (1% O 2 ) conditions. Results are presented as the percentage of cells in G0/1, S, or G2/M phases. Data represent the mean ± SD from three independent experiments (n = 3). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The 2A3 cell line (CRL-3212, ATCC, Manassas, VA, USA) was generated by transfecting FaDu cells with the E6 and E7 genes of HPV-16.

Techniques: Cell Culture, Software, Irradiation

Journal: bioRxiv

Article Title: HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation

doi: 10.1101/2025.10.26.684626

Figure Lengend Snippet: (A) Cleaved caspase-3 levels in FaDu, 2A3, and Detroit-562 cells cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Results are shown as fold change relative to non-irradiated normoxic controls. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. (B) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (C) Representative western blots of N-cadherin, E-cadherin, and vimentin in FaDu and 2A3 cells. (D) Quantification of protein levels expressed as mean optical density (O.D.) ± SD, normalized to β-actin, in FaDu and 2A3 cells under normoxic or hypoxic conditions. (E–G) Effects of hypoxia and irradiation on N-cadherin, E-cadherin, and vimentin levels in FaDu and 2A3 cells under normoxic and hypoxic conditions. Data represent mean ± SD from three independent experiments (n = 3). Statistical analysis: unpaired t-test (*p < 0.05).

Article Snippet: The 2A3 cell line (CRL-3212, ATCC, Manassas, VA, USA) was generated by transfecting FaDu cells with the E6 and E7 genes of HPV-16.

Techniques: Cell Culture, Irradiation, Software, Western Blot

Journal: bioRxiv

Article Title: HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation

doi: 10.1101/2025.10.26.684626

Figure Lengend Snippet: (A) Heatmap of the top 20 DEGs. Averaged data from three independent experiments are shown. The color bar is showing the values of z-score for each gene after library size normalization via DESeq2 software. (B) Cytokine/chemokine production in response to hypoxia, gamma-irradiation, and their combination. Conditioned media from FaDu, 2A3, and Detroit-562 cells were collected 48 hours after gamma-irradiation under normoxic or hypoxic conditions. Media from three independent experiments per cell line were pooled for analysis. Results are presented as z-scores of log 10 -transformed normalized data. n.d. = not detected. (C) Cell numbers of FaDu, 2A3, and Detroit-562 cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions, assessed 48 hours after 6 Gy gamma-irradiation. Data represent mean ± SD from four independent experiments (n = 4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. Levels of IL-8 (D), MIF (E), and Serpin E1 (F) in supernatants from FaDu, 2A3, and Detroit-562 cells cultured under normoxic or hypoxic conditions, assessed 48 hours after gamma-irradiation. Concentrations were normalized to cell numbers per condition. Data represent mean ± SD from three to four independent experiments (n = 3–4). Statistical analysis: two-way ANOVA with Tukey’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The 2A3 cell line (CRL-3212, ATCC, Manassas, VA, USA) was generated by transfecting FaDu cells with the E6 and E7 genes of HPV-16.

Techniques: Software, Irradiation, Transformation Assay, Cell Culture

Lactate dehydrogenase assay of CasKi and 2A3 cells treated in vitro with various concentrations of cisplatin.

Journal: British Journal of Cancer

Article Title: Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein

doi: 10.1038/bjc.2013.43

Figure Lengend Snippet: Lactate dehydrogenase assay of CasKi and 2A3 cells treated in vitro with various concentrations of cisplatin.

Article Snippet: The HPV16+ 2A3 cell line was produced by stably transfecting the FaDu cell line from the American Type Culture Collection with the LXSN 16E6/E7 as described in Harris et al (2011) .

Techniques: Lactate Dehydrogenase Assay, In Vitro

Tumour uptake of E6-binding mAb 188 Re-C1P5 in nude mice-bearing CasKi and 2A3 tumours post treatment with cisplatin. Mice were treated with various doses of cisplatin for 3 days, 188 Re-C1P5 mAb was administered 24 h after the last dose of cisplatin, and the tumour uptake was assessed 24 h post 188 Re-C1P5 administration: ( A ) CasKi; ( B ) 2A3; ( C , D ) TUNEL staining of the untreated and cisplatin-treated tumours. Apoptotic cells stained brown. Original magnification × 400: ( C ) CasKi tumours—untreated (left panel) and treated with 5 mg kg −1 cisplatin (right panel); ( D ) 2A3 tumours—untreated (left panel) and treated with 5 mg kg −1 cisplatin (right panel).

Journal: British Journal of Cancer

Article Title: Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein

doi: 10.1038/bjc.2013.43

Figure Lengend Snippet: Tumour uptake of E6-binding mAb 188 Re-C1P5 in nude mice-bearing CasKi and 2A3 tumours post treatment with cisplatin. Mice were treated with various doses of cisplatin for 3 days, 188 Re-C1P5 mAb was administered 24 h after the last dose of cisplatin, and the tumour uptake was assessed 24 h post 188 Re-C1P5 administration: ( A ) CasKi; ( B ) 2A3; ( C , D ) TUNEL staining of the untreated and cisplatin-treated tumours. Apoptotic cells stained brown. Original magnification × 400: ( C ) CasKi tumours—untreated (left panel) and treated with 5 mg kg −1 cisplatin (right panel); ( D ) 2A3 tumours—untreated (left panel) and treated with 5 mg kg −1 cisplatin (right panel).

Techniques: Binding Assay, TUNEL Assay, Staining

Therapy with cisplatin and RIT with E6-binding mAb 188 Re-C1P5 of nude mice-bearing 2A3 ( A ) and CasKi ( B ) tumours. T n—tumour volume on the day of measurement; T 0 —tumour volume on day 0. The mice with 2A3 HNSCC tumours were treated IP with: 400 μ Ci 188 Re-C1P5 mAb on day 0; or 5 mg kg −1 per day cisplatin on days 0, 1, 2 followed by 400 μ Ci 188 Re-C1P5 mAb on day 3; or 5 mg kg −1 cisplatin alone on days 0, 1, 2; or left untreated. The mice with CasKi cervical tumours were treated IP with: 200 μ Ci 188 Re-C1P5 mAb on day 0; or 5 mg kg −1 per day cisplatin on days 0, 1, 2 followed by 200 μ Ci 188 Re-C1P5 mAb on day 3; or 5 mg kg −1 cisplatin alone on days 0. 1, 2; or left untreated.

Journal: British Journal of Cancer

Article Title: Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein

doi: 10.1038/bjc.2013.43

Figure Lengend Snippet: Therapy with cisplatin and RIT with E6-binding mAb 188 Re-C1P5 of nude mice-bearing 2A3 ( A ) and CasKi ( B ) tumours. T n—tumour volume on the day of measurement; T 0 —tumour volume on day 0. The mice with 2A3 HNSCC tumours were treated IP with: 400 μ Ci 188 Re-C1P5 mAb on day 0; or 5 mg kg −1 per day cisplatin on days 0, 1, 2 followed by 400 μ Ci 188 Re-C1P5 mAb on day 3; or 5 mg kg −1 cisplatin alone on days 0, 1, 2; or left untreated. The mice with CasKi cervical tumours were treated IP with: 200 μ Ci 188 Re-C1P5 mAb on day 0; or 5 mg kg −1 per day cisplatin on days 0, 1, 2 followed by 200 μ Ci 188 Re-C1P5 mAb on day 3; or 5 mg kg −1 cisplatin alone on days 0. 1, 2; or left untreated.

Techniques: Binding Assay

18 F-FDG microPET/CT images of 2A3 tumour-bearing on day 15 post treatment: left panel—untreated mouse, middle panel—the mouse treated with cisplatin alone, right panel—the mouse treated with cisplatin and RIT. Green arrows are pointing to the tumours.

Journal: British Journal of Cancer

Article Title: Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein

doi: 10.1038/bjc.2013.43

Figure Lengend Snippet: 18 F-FDG microPET/CT images of 2A3 tumour-bearing on day 15 post treatment: left panel—untreated mouse, middle panel—the mouse treated with cisplatin alone, right panel—the mouse treated with cisplatin and RIT. Green arrows are pointing to the tumours.

Techniques:

Immunohistochemical evaluation of E6 and E7 oncogenes expression in RIT-treated CasKi and 2A3 cells. ( A ) E6 in CasKi cells, ( B ) E7 in CasKi cells; ( C ) E6 in 2A3 cells; ( D ) E7 in 2A3 cells. Left panels show untreated cells and right panels RIT-treated cells. Cells positive for E6 and E7 oncogenes stained brown. Original magnification × 400.

Journal: British Journal of Cancer

Article Title: Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein

doi: 10.1038/bjc.2013.43

Figure Lengend Snippet: Immunohistochemical evaluation of E6 and E7 oncogenes expression in RIT-treated CasKi and 2A3 cells. ( A ) E6 in CasKi cells, ( B ) E7 in CasKi cells; ( C ) E6 in 2A3 cells; ( D ) E7 in 2A3 cells. Left panels show untreated cells and right panels RIT-treated cells. Cells positive for E6 and E7 oncogenes stained brown. Original magnification × 400.

Techniques: Immunohistochemical staining, Expressing, Staining

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1) Product Images from "HPV status and oxygen tension shape transcriptomic, inflammatory, and cell cycle responses in HNSCC treated with ionizing radiation"

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